{"id":2383,"date":"2013-08-22T11:15:37","date_gmt":"2013-08-22T11:15:37","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=2383"},"modified":"2013-08-22T11:15:37","modified_gmt":"2013-08-22T11:15:37","slug":"article-nucleic-acids-research","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=2383","title":{"rendered":"Article: Nucleic Acids Research"},"content":{"rendered":"<p><a href=\"http:\/\/scholar.google.fr\/citations?view_op=view_citation&amp;hl=fr&amp;user=LzErFMkAAAAJ&amp;sortby=pubdate&amp;citation_for_view=LzErFMkAAAAJ:4TOpqqG69KYC\">Functional assignment of KEOPS\/EKC complex subunits in the biosynthesis of the universal t6A tRNA modification<\/a><br \/>\nL Perrochia, D Guetta, A Hecker, P Forterre, T Basta<br \/>\nNucleic Acids Research<\/p>\n<h2>Abstract<\/h2>\n<p id=\"p-3\" style=\"text-align: justify;\">N<sup>6<\/sup>-threonylcarbamoyladenosine (t<sup>6<\/sup>A) is a universal tRNA modification essential for normal cell growth and accurate translation. In Archaea and Eukarya, the universal protein Sua5 and the conserved KEOPS\/EKC complex together catalyze t<sup>6<\/sup>A biosynthesis. The KEOPS\/EKC complex is composed of Kae1, a universal metalloprotein belonging to the ASHKA superfamily of ATPases; Bud32, an atypical protein kinase and two small proteins, Cgi121 and Pcc1. In this study, we investigated the requirement and functional role of KEOPS\/EKC subunits for biosynthesis of t<sup>6<\/sup>A. We demonstrated that Pcc1, Kae1 and Bud32 form a minimal functional unit, whereas Cgi121 acts as an allosteric regulator. We confirmed that Pcc1 promotes dimerization of the KEOPS\/EKC complex and uncovered that together with Kae1, it forms the tRNA binding core of the complex. Kae1 binds\u00a0L-threonyl-carbamoyl-AMP intermediate in a metal-dependent fashion and transfers the\u00a0L-threonyl-carbamoyl moiety to substrate tRNA. Surprisingly, we found that Bud32 is regulated by Kae1 and does not function as a protein kinase but as a P-loop ATPase possibly involved in tRNA dissociation. Overall, our data support a mechanistic model in which the final step in the biosynthesis of t<sup>6<\/sup>A relies on a strictly catalytic component, Kae1, and three partner proteins necessary for dimerization, tRNA binding and regulation.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Functional assignment of KEOPS\/EKC complex subunits in the biosynthesis of the universal t6A tRNA modification L Perrochia, D Guetta, A Hecker, P Forterre, T Basta Nucleic Acids Research Abstract N6-threonylcarbamoyladenosine (t6A) is a universal tRNA modification essential for normal cell &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=2383\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-2383","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/2383","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2383"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/2383\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2383"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2383"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2383"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}