{"id":2425,"date":"2013-09-17T06:41:47","date_gmt":"2013-09-17T06:41:47","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=2425"},"modified":"2013-09-17T14:23:39","modified_gmt":"2013-09-17T14:23:39","slug":"articlejacs","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=2425","title":{"rendered":"Article: JACS"},"content":{"rendered":"
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Monothiol Glutaredoxins Can Bind Linear [Fe 3 S 4]+ and [Fe 4 S 4] 2+ Clusters in Addition to [Fe 2 S 2] 2+ Clusters: Spectroscopic Characterization and Functional<\/a>\u00a0Implications<\/a><\/h1>\n
Bo Zhang<\/a>\u00a0,\u00a0Sibali Bandyopadhyay<\/a>\u00a0,\u00a0Priyanka Shakamuri<\/a>\u00a0,\u00a0Sunil G. Naik<\/a>\u00a0,\u00a0Boi Hanh Huynh<\/a>\u00a0,J\u00e9r\u00e9my Couturier<\/a>\u00a0,\u00a0Nicolas Rouhier<\/a>\u00a0, and\u00a0Michael Kenneth Johnson<\/a><\/div>\n
J. Am. Chem. Soc.<\/cite>, Just Accepted Manuscript<\/div>\n
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DOI:\u00a0<\/strong>10.1021\/ja407059n<\/div>\n<\/div>\n
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Abstract:<\/div>\n
Saccharomyces cerevisiae mitochondrial glutaredoxin 5 (Grx5) is the archetypical member of a ubiquitous class of monothiol glutaredoxins with a strictly conserved CGFS active-site sequence that has been shown to function in biological [Fe<sub>2<\/sub>S<sub>2<\/sub>]<sup>2+<\/sup> cluster trafficking. In this work, we show that recombinant S. cerevisiae Grx5 purified aerobically after prolonged exposure of the cell-free extract to air or after anaerobic reconstitution in the presence of glutathione, predominantly contains a linear [Fe<sup>3<\/sub>S<sub>4<\/sub>]<sup>+<\/sup> cluster. The excited state electronic properties and ground state electronic and vibrational properties of the linear [Fe<sup>3<\/sub>S<sub>4<\/sub>]<sup>+<\/sup> cluster have been characterized using UV-visible absorption\/CD\/MCD, EPR, M\u00f6ssbauer and resonance Raman spectroscopies. The results reveal a rhombic S = 5\/2 linear [Fe<sup>3<\/sub>S<sub>4<\/sub>]<sup>+<\/sup> cluster with properties similar to those reported for synthetic linear [Fe<sup>3<\/sub>S<sub>4<\/sub>]<sup>+<\/sup> clusters and the linear [Fe<sup>3<\/sub>S<sub>4<\/sub>]<sup>+<\/sup> clusters in purple aconitase. Moreover, the results indicate that the Fe-S cluster content previously reported for many monothiol Grxs has been misinterpreted exclusively in terms of [Fe<sub>2<\/sub>S<sub>2<\/sub>]<sup>2+<\/sup> clusters, rather than linear [Fe<sup>3<\/sub>S<sub>4<\/sub>]<sup>+<\/sup> clusters or mixtures of linear [Fe<sup>3<\/sub>S<sub>4<\/sub>]<sup>+<\/sup> and [Fe<sub>2<\/sub>S<sub>2<\/sub>]<sup>2+<\/sup> clusters. In the absence of GSH, anaerobic reconstitution of Grx5 yields a dimeric form containing one [Fe<sub>4<\/sub>S<sub>4<\/sub>]<sup>2+<\/sup> cluster that is competent for in vitro activation of apo-aconitase, via intact cluster transfer. The ligation of the linear [Fe<sup>3<\/sub>S<sub>4<\/sub>]<sup>+<\/sup> and [Fe<sub>4<\/sub>S<sub>4<\/sub>]<sup>2+<\/sup> clusters in Grx5 has been assessed by spectroscopic, mutational and analytical studies. Potential roles for monothiol Grx5 in scavenging and recycling linear [Fe<sup>3<\/sub>S<sub>4<\/sub>]<sup>+<\/sup> clusters released during protein unfolding under oxidative stress conditions and in maturation of [Fe<sub>4<\/sub>S<sub>4<\/sub>]<sup>2+<\/sup> cluster-containing proteins are discussed in light of these results.<\/div>\n<\/div>\n
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Monothiol Glutaredoxins Can Bind Linear [Fe 3 S 4]+ and [Fe 4 S 4] 2+ Clusters in Addition to [Fe 2 S 2] 2+ Clusters: Spectroscopic Characterization and Functional\u00a0Implications Bo Zhang\u00a0,\u00a0Sibali Bandyopadhyay\u00a0,\u00a0Priyanka Shakamuri\u00a0,\u00a0Sunil G. Naik\u00a0,\u00a0Boi Hanh Huynh\u00a0,J\u00e9r\u00e9my Couturier\u00a0,\u00a0Nicolas Rouhier\u00a0, and\u00a0Michael … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-2425","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/2425","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2425"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/2425\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2425"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2425"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}