{"id":2957,"date":"2013-11-12T10:28:02","date_gmt":"2013-11-12T10:28:02","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=2957"},"modified":"2013-11-12T10:28:23","modified_gmt":"2013-11-12T10:28:23","slug":"article-molecular-plant","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=2957","title":{"rendered":"Article: Molecular Plant"},"content":{"rendered":"<p><a href=\"http:\/\/mplant.oxfordjournals.org\/content\/early\/2013\/11\/06\/mp.sst156.short\">Monothiol glutaredoxin-BolA interactions: redox control of Arabidopsis thaliana BolA2 and SufE1<\/a><\/p>\n<p>J Couturier, HC Wu, T Dhalleine, H P\u00e9geot, D Sudre\u2026 &#8211; Molecular Plant, 2013.<\/p>\n<h2>Abstract<\/h2>\n<p id=\"p-2\" style=\"text-align: justify;\">A functional relationship between monothiol glutaredoxins and BolA has been unravelled by genomic analyses and in several high-throughput studies. Phylogenetic analyses coupled to transient expression of GFP fusions indicated that, in addition to the sulfurtransferase SufE1, which contains a C-terminal BolA domain, three BolA isoforms exist in<em>Arabidopsis thaliana<\/em>, BolA1 being plastidial, BolA2 nucleo-cytoplasmic and BolA4 dual-targeted to mitochondria and plastids. Binary yeast-two hybrid experiments demonstrated that all BolAs and SufE1, via its BolA domain, can interact with all monothiol glutaredoxins. Most interactions between protein couples of the same sub-cellular compartment have been confirmed by bimolecular fluorescence complementation.\u00a0<em>In vitro<\/em>experiments indicated that monothiol glutaredoxins could regulate the redox state of BolA2 and SufE1, both proteins possessing a single conserved reactive cysteine. Indeed, a glutathionylated form of SufE1 lost its capacity to activate the cysteine desulfurase, Nfs2, but it is reactivated by plastidial glutaredoxins. Besides, a monomeric glutathionylated form and a dimeric disulfide-bridged form of BolA2 can be preferentially reduced by the nucleo-cytoplasmic GrxS17. These results indicate that the glutaredoxin-BolA interaction occurs in several sub-cellular compartments and suggest that a redox regulation mechanism, disconnected from their capacity to form iron-sulfur cluster-bridged heterodimers, may be physiologically relevant for BolA2 and SufE1.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Monothiol glutaredoxin-BolA interactions: redox control of Arabidopsis thaliana BolA2 and SufE1 J Couturier, HC Wu, T Dhalleine, H P\u00e9geot, D Sudre\u2026 &#8211; Molecular Plant, 2013. Abstract A functional relationship between monothiol glutaredoxins and BolA has been unravelled by genomic analyses &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=2957\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-2957","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/2957","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2957"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/2957\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2957"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2957"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2957"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}