{"id":3770,"date":"2014-05-19T17:41:30","date_gmt":"2014-05-19T17:41:30","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=3770"},"modified":"2014-05-19T17:43:07","modified_gmt":"2014-05-19T17:43:07","slug":"seminar-joy-watts","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=3770","title":{"rendered":"Seminar: Joy Watts"},"content":{"rendered":"<div>\n<h1><strong>\u00a0Tue may 27th ,\u00a0\u00a02.30pm, conference room INRA (SDAR building)<\/strong><\/h1>\n<h1><strong><a href=\"http:\/\/www.port.ac.uk\/school-of-biological-sciences\/staff\/dr-joy-watts.html\">Joy Watts<\/a><\/strong> (University of Portsmouth, UK)<\/h1>\n<\/div>\n<div style=\"text-align: center;\"><strong>\u00ab\u00a0Bacterial diversity, nitrogen fixation and lignocellulose composition changes in gastrointestinal tracts of a wood eating catfish&#8221;<\/strong><\/div>\n<div>\n<p style=\"text-align: justify;\">The armoured catfish, <i style=\"text-align: justify;\">Panaque nigrolineatus<\/i>, has specialized physiological adaptations to enable high levels of wood to be ingested in its diet. However, it is unclear what nutritional benefit is derived or the nature of any symbiosis that facilitates it.\u00a0 In the present study, we examined microbial communities associated with the foregut, midgut, hindgut, and auxiliary lobe of <i style=\"text-align: justify;\">P.<\/i> <i style=\"text-align: justify;\">nigrolineatus <\/i>using a metagenomic approach utilizing 454 pyrosequencing.\u00a0 Microbial community diversity altered in the different regions of the GI tract indicating different functional roles of key bacterial populations.\u00a0 To complement this genetic approach, scanning electron microscopy of the different regions of the gastrointestinal tract was performed to examine the microbial community present.\u00a0 Lignocellulose degradation within the gastrointestinal tract was supported by scanning electron microscopy observations, indicating structural alterations in the wood.\u00a0 Furthermore, the wood particles in the hindgut appeared to harbour assemblies suggestive of microbial cells. \u00a0Additionally, wood collected from different regions of the GI tract was analyzed using Fourier-Transformed Infrared (FTIR) spectroscopy.\u00a0 This technique revealed changes to the wood associated with gut transit.\u00a0 These changes are consistent with the presence of a wood-digesting activity within the GI tract within the <i style=\"text-align: justify;\">P. nigrolineatus<\/i> gastrointestinal tract.\u00a0 Furthermore, using fixed GI tract tissue using catalyzed reporter deposition enhanced immunofluorescence revealed the highest densities of nitrogenase-containing cells attached to the woody digesta within the GI tract with fewer cells sparsely colonizing the intestinal mucous layer.\u00a0 These findings are consistent with a role for the <i style=\"text-align: justify;\">P. nigrolineatus<\/i> intestinal tract microbial community in providing a source of fixed nitrogen under nitrogen limiting conditions.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0Tue may 27th ,\u00a0\u00a02.30pm, conference room INRA (SDAR building) Joy Watts (University of Portsmouth, UK) \u00ab\u00a0Bacterial diversity, nitrogen fixation and lignocellulose composition changes in gastrointestinal tracts of a wood eating catfish&#8221; The armoured catfish, Panaque nigrolineatus, has specialized physiological adaptations &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=3770\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-3770","post","type-post","status-publish","format-standard","hentry","category-seminarmeeting"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/3770","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3770"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/3770\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3770"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3770"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3770"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}