{"id":4104,"date":"2014-09-04T05:42:14","date_gmt":"2014-09-04T05:42:14","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4104"},"modified":"2014-09-04T05:45:27","modified_gmt":"2014-09-04T05:45:27","slug":"article-acta-crystallographica","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4104","title":{"rendered":"Article: Acta Crystallographica"},"content":{"rendered":"<p><a href=\"http:\/\/scripts.iucr.org\/cgi-bin\/paper?kw5097\">X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana<\/a><\/p>\n<div>T Roret, H P\u00e9geot, J Couturier, G Mulliert, N Rouhier, C Didierjean<\/div>\n<div>Acta Crystallographica Section F: Structural Biology Communications 70 (9 &#8230;<\/div>\n<p style=\"text-align: justify;\"><strong>Abstract:<\/strong><\/p>\n<p style=\"text-align: justify;\">The chloroplastic\u00a0<i>Arabidopsis thaliana<\/i>\u00a0Nfs2 (AtNfs2) is a group II pyridoxal 5&#8242;-phosphate-dependent cysteine desulfurase that is involved in the initial steps of iron-sulfur cluster biogenesis. The group II cysteine desulfurases require the presence of sulfurtransferases such as SufE proteins for optimal activity. Compared with group I cysteine desulfurases, proteins of this group contains a smaller extended lobe harbouring the catalytic cysteine and have a\u00a0<img decoding=\"async\" alt=\"[beta]\" src=\"http:\/\/scripts.iucr.org\/logos\/entities\/beta_rmgif.gif\" border=\"0\" \/>-hairpin constraining the active site. Here, two crystal structures of AtNfs2 are reported: a wild-type form with the catalytic cysteine in a persulfide-intermediate state and a C384S variant mimicking the resting state of the enzyme. In both structures the well conserved Lys241 covalently binds pyridoxal 5&#8242;-phosphate, forming an internal aldimine. Based on available homologous bacterial complexes, a model of a complex between AtNfs2 and the SufE domain of its biological partner AtSufE1 is proposed, revealing the nature of the binding sites.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana T Roret, H P\u00e9geot, J Couturier, G Mulliert, N Rouhier, C Didierjean Acta Crystallographica Section F: Structural Biology Communications 70 (9 &#8230; Abstract: The chloroplastic\u00a0Arabidopsis thaliana\u00a0Nfs2 (AtNfs2) is a &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4104\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-4104","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/4104","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4104"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/4104\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4104"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4104"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4104"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}