{"id":4126,"date":"2014-09-08T15:51:10","date_gmt":"2014-09-08T15:51:10","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4126"},"modified":"2014-09-08T15:51:10","modified_gmt":"2014-09-08T15:51:10","slug":"article-fungal-genetics-and-biology-2","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4126","title":{"rendered":"Article: Fungal Genetics and Biology"},"content":{"rendered":"<p style=\"text-align: justify;\"><a href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S1087184514001388\"><em>OmGOGAT<\/em>\u00a0disruption in the ericoid mycorrhizal fungus <em>Oidiodendron<\/em> <em>maius<\/em> induces reorganization of the N pathway and reduces tolerance to heavy-metals<\/a>.\u00a0HR Khouja, S Daghino, S Abb\u00e0, F Boutaraa, M Chalot, D Blaudez, \u2026Fungal Genetics and Biology<\/p>\n<div><\/div>\n<h2 id=\"authorab0101\">Abstract<\/h2>\n<p id=\"sp0010\" style=\"text-align: justify;\">Mycorrhizal fungi are key mediators of soil-to-plant movement of mineral nutrients, including essential and non-essential metals. In soil conditions that facilitate mobilization of metal ions, potentially toxic metals can interfere with nitrogen metabolism in both plants and microorganisms. Less is known about possible relationships between nitrogen metabolism and responses to heavy metals. Aim of this study was to investigate this aspect in the ericoid mycorrhizal fungus\u00a0<em>Oidiodendron maius<\/em>\u00a0strain Zn, a metal tolerant ascomycete. Growth of\u00a0<em>O. maius<\/em>\u00a0Zn on zinc and cadmium containing media was significantly affected by the nitrogen source. Screening of a library of\u00a0<em>O. maius<\/em>\u00a0Zn random genetic transformants for sensitivity to heavy metals (zinc and cadmium) and oxidative stress (menadione) yielded a mutant strain that carried a partial deletion of the glutamate synthase (NADH-GOGAT EC 1.4.1.14) gene and its adjacent gene, the\u00a0<em>APC15<\/em>subunit of the anaphase promoting complex. Comparison of WT and\u00a0<em>OmGOGAT-OmAPC15<\/em>\u00a0mutant strains indicated an impaired N-metabolism and altered stress tolerance, and assays on the\u00a0<em>OmAPC15<\/em>-recomplemented strains ascribed the observed phenotypes to the deletion in the\u00a0<em>OmGOGAT<\/em>\u00a0gene.<em>OmGOGAT<\/em>\u00a0disruption modified the nitrogen pathway, with a strong reduction of the associated glutamine synthetase (GS, EC 6.3.1.2) activity and an up-regulation of the alternative NADP-glutamate dehydrogenase (NADP-GDH, EC 1.4.1.4) pathway for glutamate biosynthesis. Unless they were supplemented with glutamine,\u00a0<em>O. maius<\/em>\u00a0Zn transformants lacking\u00a0<em>OmGOGAT<\/em>\u00a0were very sensitive to zinc. These results highlight the importance of nitrogen metabolism not only for nitrogen assimilation and transformation, but also for stress tolerance. For mycorrhizal fungi, such as\u00a0<em>O. maius<\/em>, this may bear consequences not only to the fungus, but also to the host plant.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>OmGOGAT\u00a0disruption in the ericoid mycorrhizal fungus Oidiodendron maius induces reorganization of the N pathway and reduces tolerance to heavy-metals.\u00a0HR Khouja, S Daghino, S Abb\u00e0, F Boutaraa, M Chalot, D Blaudez, \u2026Fungal Genetics and Biology Abstract Mycorrhizal fungi are key mediators &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4126\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-4126","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/4126","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4126"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/4126\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4126"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4126"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4126"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}