{"id":4810,"date":"2015-03-06T12:29:39","date_gmt":"2015-03-06T12:29:39","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4810"},"modified":"2015-03-06T12:39:17","modified_gmt":"2015-03-06T12:39:17","slug":"article-fungal-genetics-and-biology-3","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4810","title":{"rendered":"Article: Fungal Genetics and Biology"},"content":{"rendered":"

Evidence for homoploid speciation in Phytophthora alni supports taxonomic reclassification in this species complex<\/a><\/p>\n

C Husson, J Aguayo, C Revellin, P Frey, R Ioos, B Mar\u00e7ais<\/div>\n
Fungal Genetics and Biology<\/div>\n

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Abstract<\/h2>\n

Alder decline has been a problem along European watercourses since the early 1990s. Hybridization was identified as the main cause of this emerging disease. Indeed, the causal agent, a soil-borne pathogen named\u00a0Phytophthora alni<\/em>\u00a0subsp.\u00a0alni (Paa)<\/em>\u00a0is the result of interspecific hybridization between two taxa,\u00a0Phytophthora alni<\/em>\u00a0subsp.\u00a0multiformis (Pam)<\/em>\u00a0and\u00a0Phytophthora alni<\/em>\u00a0subsp.\u00a0uniformis (Pau)<\/em>, initially identified as subspecies of\u00a0Paa<\/em>. The aim of this work was to characterize the ploidy level within the\u00a0P. alni<\/em>\u00a0complex that is presently poorly understood. For that, we used two complementary approaches for a set of 31 isolates of\u00a0Paa<\/em>,\u00a0Pam<\/em>\u00a0and\u00a0Pau<\/em>: (i) quantification of allele copy number of three single-copy nuclear genes using allele-specific real-time PCR and (ii) comparison of the genome size estimated by flow cytometry. Relative quantification of alleles of the three single-copy genes showed that the copy number of a given allele in\u00a0Paa<\/em>\u00a0was systematically half that of its parents\u00a0Pau<\/em>\u00a0or\u00a0Pam<\/em>. Moreover, DNA content estimated by flow cytometry in\u00a0Paa<\/em>\u00a0was equal to half the sum of those in\u00a0Pam<\/em>\u00a0and\u00a0Pau<\/em>. Our results therefore suggest that the hybrid\u00a0Paa<\/em>\u00a0is an allotriploid species, containing half of the genome of each of its parents\u00a0Pam<\/em>\u00a0and\u00a0Pau<\/em>, which in turn are considered to be allotetraploid and diploid, respectively.\u00a0Paa<\/em>\u00a0thus results from a homoploid speciation process. Based on published data and on results from this study, a new formal taxonomic name is proposed for the three taxa\u00a0Paa<\/em>,\u00a0Pam<\/em>\u00a0and\u00a0Pau<\/em>\u00a0which are raised to species status and renamed\u00a0P.<\/em>\u00a0\u00d7alni<\/em>,\u00a0P.<\/em>\u00a0\u00d7multiformis<\/em>\u00a0and\u00a0P. uniformis<\/em>, respectively.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Evidence for homoploid speciation in Phytophthora alni supports taxonomic reclassification in this species complex C Husson, J Aguayo, C Revellin, P Frey, R Ioos, B Mar\u00e7ais Fungal Genetics and Biology Abstract Alder decline has been a problem along European watercourses … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-4810","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/4810","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4810"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/4810\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4810"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4810"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4810"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}