{"id":4831,"date":"2015-03-10T15:26:07","date_gmt":"2015-03-10T15:26:07","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4831"},"modified":"2015-03-10T15:27:50","modified_gmt":"2015-03-10T15:27:50","slug":"article-environmental-microbiology-3","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4831","title":{"rendered":"Article: Environmental Microbiology"},"content":{"rendered":"<p style=\"text-align: justify;\"><a class=\"gsc_a_at\" href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.12840\/abstract\">Metagenome sequence of Elaphomyces granulatus from sporocarp tissue reveals Ascomycota ectomycorrhizal fingerprints of genome expansion and a Proteobacteria rich microbiome<\/a><\/p>\n<div class=\"gs_gray\">CA Quandt, A Kohler, C Hesse, T Sharpton, F Martin, JW Spatafora<\/div>\n<div class=\"gs_gray\">Environmental Microbiology<\/div>\n<div class=\"gs_gray\"><\/div>\n<div class=\"gs_gray\">\n<h1><\/h1>\n<h1>Abstract<\/h1>\n<div class=\"para\">\n<p style=\"text-align: justify;\">Many obligate symbiotic fungi are difficult to maintain in culture, and there is a growing need for alternative approaches to obtaining tissue and subsequent genomic assemblies from such species. In this study, the genome of\u00a0<em>Elaphomyces granulatus<\/em>\u00a0was sequenced from sporocarp tissue. The genome assembly remains on many contigs, but gene space is estimated to be mostly complete. Phylogenetic analyses revealed that the\u00a0<em>Elaphomyces<\/em>\u00a0lineage is most closely related to\u00a0<em>Talaromyces<\/em>\u00a0and Trichocomaceae s.s. The genome of\u00a0<em>E. granulatu<\/em>s is reduced in CAZymes, despite a large expansion in genome size, both of which are consistent with what is seen in\u00a0<em>T. melanosporum<\/em>, the other sequenced ectomycorrhizal ascomycete. A large number of transposable elements are predicted in the\u00a0<em>E. granulatus<\/em>\u00a0genome, especially Gypsy-like long terminal repeats (LTR), and there has also been an expansion in helicases. The metagenome is a complex community dominated by bacteria in Bradyrhizobiaceae, and there is evidence to suggest that the community may be reduced in functional capacity as estimated by KEGG pathways. Through the sequencing of sporocarp tissue this study has provided insights into\u00a0<em>Elaphomyces<\/em>\u00a0phylogenetics, genomics, metagenomics, and the evolution of the ectomycorrhizal association.<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Metagenome sequence of Elaphomyces granulatus from sporocarp tissue reveals Ascomycota ectomycorrhizal fingerprints of genome expansion and a Proteobacteria rich microbiome CA Quandt, A Kohler, C Hesse, T Sharpton, F Martin, JW Spatafora Environmental Microbiology Abstract Many obligate symbiotic fungi are &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4831\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-4831","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/4831","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4831"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/4831\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4831"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4831"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4831"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}