{"id":4837,"date":"2015-03-10T15:33:41","date_gmt":"2015-03-10T15:33:41","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4837"},"modified":"2015-03-10T15:33:41","modified_gmt":"2015-03-10T15:33:41","slug":"article-journal-of-experimental-botany","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4837","title":{"rendered":"Article: Journal of Experimental Botany"},"content":{"rendered":"<p><a class=\"gsc_a_at\" href=\"http:\/\/jxb.oxfordjournals.org\/content\/early\/2015\/03\/03\/jxb.erv066.abstract\">Function of glutathione peroxidases in legume root nodules<\/a><\/p>\n<div class=\"gs_gray\">MA Matamoros, A Saiz, M Pe\u00f1uelas, P Bustos-Sanmamed, JM Mulet, &#8230;<\/div>\n<div class=\"gs_gray\">Journal of Experimental Botany, erv066<\/div>\n<div class=\"gs_gray\"><\/div>\n<div class=\"gs_gray\">\n<h2 style=\"text-align: justify;\">Abstract<\/h2>\n<p id=\"p-3\" style=\"text-align: justify;\">Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far in legume nodules, despite the fact that reactive oxygen species are produced at different steps of the symbiosis. The function of two Gpxs that are highly expressed in nodules of the model legume\u00a0<em>Lotus japonicus<\/em>was examined. Gene expression analysis, enzymatic and nitrosylation assays, yeast cell complementation,\u00a0<em>in situ<\/em>\u00a0mRNA hybridization, immunoelectron microscopy, and LjGpx-green fluorescent protein (GFP) fusions were used to characterize the enzymes and to localize each transcript and isoform in nodules. The\u00a0<em>LjGpx1<\/em>\u00a0and\u00a0<em>LjGpx3<\/em>\u00a0genes encode thioredoxin-dependent phospholipid hydroperoxidases and are differentially regulated in response to nitric oxide (NO) and hormones. LjGpx1 and LjGpx3 are nitrosylated\u00a0<em>in vitro<\/em>\u00a0or in plants treated with\u00a0<em>S<\/em>-nitrosoglutathione (GSNO). Consistent with the modification of the peroxidatic cysteine of LjGpx3,\u00a0<em>in vitro<\/em>\u00a0assays demonstrated that this modification results in enzyme inhibition. The enzymes are highly expressed in the infected zone, but the LjGpx3 mRNA is also detected in the cortex and vascular bundles. LjGpx1 is localized to the plastids and nuclei, and LjGpx3 to the cytosol and endoplasmic reticulum. Based on yeast complementation experiments, both enzymes protect against oxidative stress, salt stress, and membrane damage. It is concluded that both LjGpxs perform major antioxidative functions in nodules, preventing lipid peroxidation and other oxidative processes at different subcellular sites of vascular and infected cells. The enzymes are probably involved in hormone and NO signalling, and may be regulated through nitrosylation of the peroxidatic cysteine essential for catalytic function.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Function of glutathione peroxidases in legume root nodules MA Matamoros, A Saiz, M Pe\u00f1uelas, P Bustos-Sanmamed, JM Mulet, &#8230; Journal of Experimental Botany, erv066 Abstract Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far in legume nodules, despite the &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=4837\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-4837","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/4837","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4837"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/4837\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4837"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4837"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4837"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}