{"id":5154,"date":"2015-06-15T11:44:22","date_gmt":"2015-06-15T11:44:22","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=5154"},"modified":"2015-06-15T11:44:29","modified_gmt":"2015-06-15T11:44:29","slug":"article-plant-pathology-2","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=5154","title":{"rendered":"Article: Plant Pathology"},"content":{"rendered":"<p><a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ppa.12426\/abstract;jsessionid=DBD9166985DF5DAE5646178AD8A4AD6E.f01t02\">Simultaneous monitoring and quantification of Melampsora allii-populina and Melampsora larici-populina on infected poplar leaves using a duplex real-time PCR assay.<\/a>\u00a0C. Guinet, AL Boutigny, A Vialle, RC Hammelin, P Frey, R Ioos. Plant pathology<\/p>\n<h1>Abstract<\/h1>\n<div class=\"para\">\n<p style=\"text-align: justify;\">The rust fungi\u00a0<em>Melampsora larici-populina<\/em>\u00a0(<em>Mlp<\/em>) and\u00a0<em>Melampsora allii-populina<\/em>\u00a0(<em>Map<\/em>) are the main phytosanitary constraints for commercial poplar cultivation in Europe. Although\u00a0<em>Mlp<\/em>\u00a0is more aggressive and prevalent than\u00a0<em>Map<\/em>, the two species may co-infect the same poplar tree or even the same poplar leaf, making the epidemiological surveys of each species difficult to achieve. In this study, a new duplex real-time PCR assay targeting each species was developed, based on single-copy genes. This test proved to be specific, inclusive and was successfully used to detect and quantify each species, starting from urediniospore samples or directly from infected poplar leaves, with or without visible symptoms. This new molecular tool was also assessed for comparative studies of time-course infection experiments on artificially inoculated poplar leaf discs. These studies showed that the growth dynamics of\u00a0<em>Map<\/em>\u00a0was significantly slower when the two species were co-inoculated on the same leaf disc, confirming that\u00a0<em>Map<\/em>\u00a0is less aggressive than\u00a0<em>Mlp<\/em>.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Simultaneous monitoring and quantification of Melampsora allii-populina and Melampsora larici-populina on infected poplar leaves using a duplex real-time PCR assay.\u00a0C. Guinet, AL Boutigny, A Vialle, RC Hammelin, P Frey, R Ioos. Plant pathology Abstract The rust fungi\u00a0Melampsora larici-populina\u00a0(Mlp) and\u00a0Melampsora allii-populina\u00a0(Map) &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=5154\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-5154","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/5154","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5154"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/5154\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5154"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5154"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5154"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}