{"id":6754,"date":"2016-08-17T06:56:24","date_gmt":"2016-08-17T06:56:24","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=6754"},"modified":"2016-08-17T06:56:24","modified_gmt":"2016-08-17T06:56:24","slug":"article-plant-science","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=6754","title":{"rendered":"Article: Plant science"},"content":{"rendered":"

Plastidic P2 glucose-6P dehydrogenase from poplar is modulated by thioredoxin m-type: distinct roles of cysteine residues in redox regulation and NADPH inhibition<\/a>\u00a0M Cardi, M Zaffagnini, A De Lillo, D Castiglia, K Chibani, JM Gualberto, …Plant Science<\/p>\n

 <\/p>\n

Abstract<\/h2>\n

A cDNA coding for a plastidic P2-type G6PDH isoform from poplar (Populus tremula x tremuloides)<\/em>\u00a0has been used to express and purify to homogeneity the mature recombinant protein with a N-terminus His-tag. The study of the kinetic properties of the recombinant enzyme showed an\u00a0in vitro<\/em>\u00a0redox sensing modulation exerted by reduced DTT. The interaction with thioredoxins (TRXs) was then investigated.<\/p>\n

Five cysteine to serine variants (C145S \u2013 C175S \u2013 C183S \u2013 C195S \u2013 C242S) and a variant with a double substitution for Cys175<\/sup>\u00a0and Cys183<\/sup>\u00a0(C175S\/C183S) have been generated, purified and biochemically characterized in order to investigate the specific role(s) of cysteines in terms of redox regulation and NADPH-dependent inhibition.<\/p>\n

Three cysteine residues (C145<\/sup>, C194<\/sup>, C242<\/sup>) are suggested to have a role in controlling the NADP+<\/sup>\u00a0access to the active site, and in stabilizing the NADPH regulatory binding site.<\/p>\n

Our results also indicate that the regulatory disulfide involves residues Cys175<\/sup>\u00a0and Cys183<\/sup>\u00a0in a position similar to those of chloroplastic P1-G6PDHs, but the modulation is exerted primarily by TRX\u00a0m-<\/em>type, in contrast to P1-G6PDH, which is regulated by TRX\u00a0f<\/em>.<\/p>\n

This unexpected specificity indicates differences in the mechanism of regulation, and redox sensing of plastidic P2-G6PDH compared to chloroplastic P1-G6PDH in higher plants.<\/p>\n","protected":false},"excerpt":{"rendered":"

Plastidic P2 glucose-6P dehydrogenase from poplar is modulated by thioredoxin m-type: distinct roles of cysteine residues in redox regulation and NADPH inhibition\u00a0M Cardi, M Zaffagnini, A De Lillo, D Castiglia, K Chibani, JM Gualberto, …Plant Science   Abstract A cDNA … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-6754","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/6754","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6754"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/6754\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6754"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6754"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6754"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}