{"id":7933,"date":"2017-02-24T07:43:31","date_gmt":"2017-02-24T07:43:31","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=7933"},"modified":"2017-02-24T07:43:43","modified_gmt":"2017-02-24T07:43:43","slug":"article-nitric-oxide","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=7933","title":{"rendered":"Article: Nitric oxide"},"content":{"rendered":"<p><a class=\"gsc_a_at\" href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S1089860316302257\">Post-translational modifications of Medicago truncatula glutathione peroxidase 1 induced by nitric oxide<\/a>\u00a0C Castella, I Mirtziou, A Seassau, A Boscari, F Montrichard, &#8230; Nitric Oxide<\/p>\n<h2 id=\"authorabs00101\" class=\"secHeading\" style=\"text-align: justify;\">Abstract<\/h2>\n<p id=\"abspara0010\" style=\"text-align: justify;\">Plant glutathione peroxidases (Gpx) catalyse the reduction of various peroxides, such as hydrogen peroxide (H<sub>2<\/sub>O<sub>2<\/sub>), phospholipid hydroperoxides and peroxynitrite, but at the expense of thioredoxins rather than glutathione. A main function of plant Gpxs is the protection of biological membranes by scavenging phospholipid hydroperoxides, but some Gpxs have also been associated with H<sub>2<\/sub>O<sub>2<\/sub>\u00a0sensing and redox signal transduction. Nitric oxide (NO) is not only known to induce the expression of Gpx family members, but also to inhibit Gpx activity, presumably through the S-nitrosylation of conserved cysteine residues. In the present study, the effects of NO-donors on both the activity and S-nitrosylation state of purified\u00a0<em>Medicago truncatula<\/em>\u00a0Gpx1 were analyzed using biochemical assay measurements and a biotin-switch\/mass spectrometry approach. MtGpx1 activity was only moderately inhibited by the NO-donors diethylamine-NONOate and S-nitrosoglutathione, and the inhibition may be reversed by DTT. The three conserved Cys of MtGpx1 were found to be modified through S-nitrosylation and S-glutathionylation, although to different extents, by diethylamine-NONOate and S-nitrosoglutathione, or by a combination of diethylamine-NONOate and reduced glutathione. The regulation of MtGpx1 and its possible involvement in the signaling process is discussed in the light of these results.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Post-translational modifications of Medicago truncatula glutathione peroxidase 1 induced by nitric oxide\u00a0C Castella, I Mirtziou, A Seassau, A Boscari, F Montrichard, &#8230; Nitric Oxide Abstract Plant glutathione peroxidases (Gpx) catalyse the reduction of various peroxides, such as hydrogen peroxide (H2O2), &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=7933\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-7933","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/7933","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7933"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/7933\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7933"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7933"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7933"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}