{"id":8778,"date":"2018-03-22T16:35:08","date_gmt":"2018-03-22T16:35:08","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=8778"},"modified":"2018-03-22T16:35:08","modified_gmt":"2018-03-22T16:35:08","slug":"article-febs-letters-3","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=8778","title":{"rendered":"Article: FEBS letters"},"content":{"rendered":"<p><a class=\"gsc_a_at\" href=\"https:\/\/febs.onlinelibrary.wiley.com\/doi\/full\/10.1002\/1873-3468.13009\" data-href=\"\/citations?view_op=view_citation&amp;hl=fr&amp;user=u0KB0xMAAAAJ&amp;sortby=pubdate&amp;citation_for_view=u0KB0xMAAAAJ:Dip1O2bNi0gC\">Structural snapshots along the reaction mechanism of the atypical poplar thioredoxin\u2010like2. 1\u00a0<\/a>K Chibani, F Saul, C Didierjean, N Rouhier, A Haouz FEBS letters<\/p>\n<h1 class=\"article-section__header main abstractlang_en main\">Abstract<\/h1>\n<div class=\"article-section__content en main\">\n<p style=\"text-align: justify;\">Plastidial thioredoxin (TRX)\u2010like2.1 proteins are atypical thioredoxins possessing a WCRKC active site signature and using glutathione for recycling. To obtain structural information supporting the peculiar catalytic mechanisms and target proteins of these TRXs, we solved the crystal structures of poplar TRX\u2010like2.1 in oxidized and reduced states and of mutated variants. These structures share similar folding with TRXs exhibiting the canonical WCGPC signature. Moreover, the overall conformation is not altered by reduction of the catalytic disulfide bond or in a C45S\/C67S variant that formed a disulfide\u2010bridged dimer possibly mimicking reaction intermediates with target proteins. Modeling of the interaction of TRX\u2010like2.1 with both NADPH\u2010 and ferredoxin\u2010thioredoxin reductases (FTR) indicates that the presence of Arg43 and Lys44 residues likely precludes reduction by the plastidial FTR.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Structural snapshots along the reaction mechanism of the atypical poplar thioredoxin\u2010like2. 1\u00a0K Chibani, F Saul, C Didierjean, N Rouhier, A Haouz FEBS letters Abstract Plastidial thioredoxin (TRX)\u2010like2.1 proteins are atypical thioredoxins possessing a WCRKC active site signature and using glutathione &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=8778\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-8778","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/8778","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8778"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/8778\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8778"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8778"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8778"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}