{"id":9095,"date":"2018-07-26T15:42:18","date_gmt":"2018-07-26T15:42:18","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=9095"},"modified":"2024-02-21T08:52:44","modified_gmt":"2024-02-21T08:52:44","slug":"phd-position-on-poplar-rust","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=9095","title":{"rendered":"PhD position on poplar rust"},"content":{"rendered":"

English version: see below<\/em><\/strong><\/p>\n

This position has been filled!<\/strong><\/em><\/span><\/p>\n

Titre du sujet de th\u00e8se\u00a0:<\/strong><\/p>\n

Caract\u00e9risation \u00e9volutive et fonctionnelle d\u2019un locus d\u2019avirulence chez l\u2019agent de la rouille du peuplier<\/p>\n

Encadrants :<\/strong><\/p>\n

Pascal FREY (DR2, HDR), directeur de la th\u00e8se<\/p>\n

S\u00e9bastien Duplessis (DR2, HDR), co-directeur de la th\u00e8se<\/p>\n

Laboratoire d\u2019accueil :<\/strong><\/p>\n

UMR Interactions Arbres \u2013 Microorganismes<\/p>\n

INRA\/Universit\u00e9 de Lorraine<\/p>\n

Centre INRA Grand Est Nancy<\/p>\n

54280 Champenoux<\/p>\n

https:\/\/mycor.iam.inrae.fr\/IAM\/<\/a><\/p>\n

Universit\u00e9 d\u2019inscription de l\u2019\u00e9tudiant en th\u00e8se<\/strong>\u00a0: Universit\u00e9 de Lorraine<\/p>\n

Financement\u00a0: <\/strong>R\u00e9gion Grand Est et ANR.<\/p>\n

R\u00e9sum\u00e9 de la th\u00e8se<\/strong><\/p>\n

L\u2019objectif de la th\u00e8se propos\u00e9e est d\u2019\u00e9tudier les m\u00e9canismes sous-jacents \u00e0 l\u2019\u00e9volution de la pathog\u00e9nicit\u00e9 d\u2019un agent pathog\u00e8ne, Melampsora larici-populina<\/em>, responsable de la rouille du peuplier. Gr\u00e2ce \u00e0 une \u00e9tude d\u2019association pang\u00e9nomique (GWAS), nous avons r\u00e9cemment identifi\u00e9 un locus dans le g\u00e9nome de M. larici-populina<\/em>, qui pourrait correspondre au locus d\u2019avirulence Avr7, dont la mutation est responsable du contournement de la r\u00e9sistance R7 du peuplier. L\u2019objectif de la th\u00e8se sera d\u2019\u00e9tudier cette r\u00e9gion g\u00e9nomique et de valider fonctionnellement le locus d\u2019int\u00e9r\u00eat. Il s\u2019agirait alors du premier g\u00e8ne d\u2019avirulence identifi\u00e9 chez ce champignon. Plusieurs approches compl\u00e9mentaires sont envisag\u00e9es\u00a0: (i<\/em>) une approche de g\u00e9nomique comparative afin de pr\u00e9ciser le polymorphisme dans cette r\u00e9gion\u00a0; (ii<\/em>) une approche de transcriptomique afin de pr\u00e9ciser l\u2019expression du g\u00e8ne d\u2019avirulence et des g\u00e8nes co-exprim\u00e9s\u00a0; (iii<\/em>) une approche de g\u00e9nomique fonctionnelle pour tenter d\u2019exprimer la prot\u00e9ine correspondante dans un syst\u00e8me h\u00e9t\u00e9rologue et de rechercher son interactant dans les cellules v\u00e9g\u00e9tales. Ces approches originales, mais n\u00e9anmoins bien maitris\u00e9es par l\u2019unit\u00e9 d\u2019accueil, ont pour ambition d\u2019apporter une meilleure compr\u00e9hension de l\u2019\u00e9volution des g\u00e9nomes des agents pathog\u00e8nes afin d\u2019aider \u00e0 la d\u00e9finition de strat\u00e9gies de s\u00e9lection des plantes (en particulier le peuplier) pour une r\u00e9sistance durable.<\/p>\n

Candidature :<\/strong><\/p>\n

Envoyer un CV, une lettre de motivation et les coordonn\u00e9es de 2 personnes de r\u00e9f\u00e9rence \u00e0 Pascal Frey (pascal.frey@inra.fr<\/a>) et S\u00e9bastien Duplessis (sebastien.duplessis@inra.fr<\/a>) avant le 1er septembre 2018.<\/p>\n

Comp\u00e9tences recherch\u00e9es<\/strong><\/p>\n

Le(la) candidat(e) devra avoir de bonnes comp\u00e9tences en g\u00e9n\u00e9tique, g\u00e9nomique, bioinformatique et en biologie mol\u00e9culaire. Il(elle) devra avoir des connaissances de base en \u00e9cologie et un int\u00e9r\u00eat pour la biologie \u00e9volutive et la pathologie v\u00e9g\u00e9tale. Un go\u00fbt prononc\u00e9 pour le travail en \u00e9quipe est indispensable.<\/p>\n

————————————————————————————————–<\/p>\n

Title of the thesis<\/strong><\/p>\n

Evolutionary and functional characterization of an avirulence locus in the poplar rust fungus.<\/p>\n

Advisors:<\/strong><\/p>\n

Pascal FREY (Senior scientist), main supervisor<\/p>\n

S\u00e9bastien DUPLESSIS (Senior scientist), co-supervisor<\/p>\n

Host laboratory<\/strong><\/p>\n

Department of Tree – Microbe Interactions<\/p>\n

INRA \/ University of Lorraine<\/p>\n

INRA Grand Est Nancy research centre<\/p>\n

54280 Champenoux<\/p>\n

https:\/\/mycor.iam.inrae.fr\/IAM\/<\/a><\/p>\n

Funding :<\/strong> Region Grand Est and ANR (French National Research Agency). Duration 3 years. Monthly net salary about \u20ac1400.<\/p>\n

Thesis summary<\/strong><\/p>\n

The objective of this thesis is to study the mechanisms underlying the evolution of pathogenicity of the poplar rust fungus, Melampsora larici-populina. Through a genome-wide association study (GWAS), we recently identified a locus in the genome of M. larici-populina, which could correspond to the avirulence locus Avr7, whose mutation is responsible for R7 resistance breakdown in poplar. The objective of the thesis will be to study in fine details this genomic region and to functionally validate this avirulence locus. This would be the first avirulence gene identified in this biotrophic fungal species. Several complementary approaches will be engaged: (i) a comparative genomics approach in order to list the polymorphisms in this region; (ii) a transcriptomic approach to clarify the expression of the avirulence gene and the co-expressed genes; (iii) a functional genomic approach to attempt to express the corresponding protein in a heterologous system and to search for its interactant in plant cells. These original approaches, but nevertheless well mastered by the proposing team, aim to provide a better understanding of the evolution of the pathogen genomes in order to help define plant selection strategies for durable resistance, especially for poplar.<\/p>\n

Application<\/strong><\/p>\n

Send CV, cover letter and contact details of two referees to Pascal Frey (pascal.frey@inra.fr<\/a>) and S\u00e9bastien Duplessis (sebastien.duplessis@inra.fr<\/a>) before September 1, 2018.<\/p>\n

Required skills<\/strong><\/p>\n

The candidate should have good skills in genetics, genomics, bioinformatics and molecular biology. He\/she should have basic knowledge in ecology and an interest in evolutionary biology and plant pathology. A taste for teamwork is essential. Knowledge of French will be an asset but is not mandatory.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

English version: see below This position has been filled! Titre du sujet de th\u00e8se\u00a0: Caract\u00e9risation \u00e9volutive et fonctionnelle d\u2019un locus d\u2019avirulence chez l\u2019agent de la rouille du peuplier Encadrants : Pascal FREY (DR2, HDR), directeur de la th\u00e8se S\u00e9bastien Duplessis … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"class_list":["post-9095","post","type-post","status-publish","format-standard","hentry","category-job"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/9095","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9095"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/9095\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9095"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9095"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9095"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}