{"id":943,"date":"2013-04-23T06:14:16","date_gmt":"2013-04-23T06:14:16","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=943"},"modified":"2013-05-08T09:49:21","modified_gmt":"2013-05-08T09:49:21","slug":"new-publication-3","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=943","title":{"rendered":"Article: Fungal Biology"},"content":{"rendered":"<p><a href=\"http:\/\/scholar.google.fr\/citations?view_op=view_citation&amp;hl=fr&amp;user=qrOM-zoAAAAJ&amp;pagesize=100&amp;sortby=pubdate&amp;citation_for_view=qrOM-zoAAAAJ:35N4QoGY0k4C\">Optimization of a real-time PCR assay for the detection of the quarantine pathogen&lt; i&gt; Melampsora medusae&lt;\/i&gt; f. sp.&lt; i&gt; deltoidae&lt;\/i&gt;<\/a><br \/>\nAL Boutigny, C Guinet, A Vialle, R Hamelin, A Andrieux, P Frey, C Husson, R Ioos<br \/>\nFungal Biology<\/p>\n<p id=\"abspara0010\" style=\"text-align: justify;\"><em>Melampsora medusae<\/em>, one of the causal agents of poplar rust, is classified as an A2 quarantine pest for EPPO and its presence in Europe is strictly controlled. Two\u00a0<em>formae speciales<\/em>\u00a0have been described within\u00a0<em>M. medusae<\/em>,\u00a0<em>M. medusae<\/em>\u00a0f. sp.\u00a0<em>deltoidae<\/em>\u00a0and\u00a0<em>M. medusae<\/em>\u00a0f. sp.\u00a0<em>tremuloidae<\/em>\u00a0on the basis of their pathogenicity on\u00a0<em>Populus<\/em>\u00a0species from the section\u00a0<em>Aigeiros<\/em>\u00a0(e.g.\u00a0<em>Populus deltoides<\/em>) or\u00a0<em>Populus<\/em>\u00a0(e.g.\u00a0<em>Populus tremuloides<\/em>), respectively. In this study, a real-time PCR assay was developed allowing the detection of\u00a0<em>M. medusae<\/em>\u00a0f. sp.<em>deltoidae<\/em>, the\u00a0<em>forma specialis<\/em>\u00a0that is economically harmful. A set of primers and hydrolysis probe was designed based on sequence polymorphisms in the large ribosomal RNA subunit (<em>28S<\/em>). The real-time PCR assay was optimized and performance criteria of the detection method, i.e. sensitivity, specificity, repeatability, reproducibility and robustness, were assessed. The real-time PCR method was highly specific and sensitive and allowed the detection of one single urediniospore of\u00a0<em>M. medusae<\/em>\u00a0f. sp.\u00a0<em>deltoidae<\/em>\u00a0in a mixture of 2 mg of urediniospores of other\u00a0<em>Melampsora<\/em>\u00a0species. This test offers improved specificity over currently existing conventional PCR tests and can be used for specific surveys in European nurseries and phytosanitary controls, in order to avoid introduction and spread of this pathogen in Europe.<\/p>\n<div id=\"SD_BA1P\"><\/div>\n<h2 id=\"kwd_1\">Keywords<\/h2>\n<ul id=\"kwrds0010\">\n<li id=\"kwrd0010\"><em>Melampsora<\/em>;<\/li>\n<li id=\"kwrd0015\">poplar rust;<\/li>\n<li id=\"kwrd0020\">real-time PCR;<\/li>\n<li id=\"kwrd0025\">quarantine pathogen<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Optimization of a real-time PCR assay for the detection of the quarantine pathogen&lt; i&gt; Melampsora medusae&lt;\/i&gt; f. sp.&lt; i&gt; deltoidae&lt;\/i&gt; AL Boutigny, C Guinet, A Vialle, R Hamelin, A Andrieux, P Frey, C Husson, R Ioos Fungal Biology Melampsora medusae, &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=943\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-943","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/943","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=943"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/943\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=943"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=943"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=943"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}