{"id":9531,"date":"2019-03-01T12:48:03","date_gmt":"2019-03-01T12:48:03","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=9531"},"modified":"2019-03-01T12:48:03","modified_gmt":"2019-03-01T12:48:03","slug":"article-journal-of-experimental-botany-2","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=9531","title":{"rendered":"Article: Journal of experimental botany"},"content":{"rendered":"<p><a class=\"gsc_a_at\" href=\"https:\/\/academic.oup.com\/jxb\/advance-article-abstract\/doi\/10.1093\/jxb\/erz050\/5310294\" data-href=\"\/citations?view_op=view_citation&amp;hl=fr&amp;user=bftSCjYAAAAJ&amp;sortby=pubdate&amp;citation_for_view=bftSCjYAAAAJ:4JMBOYKVnBMC\">The iron-sulfur protein NFU2 plays a predominant role in branched-chain amino acid synthesis in Arabidopsis roots\u00a0<\/a>B Touraine, F Vignols, J Przybyla-Toscano, T Ischebeck, T Dhalleine, &#8230; Journal of experimental botany<\/p>\n<h2 id=\"131567947\" class=\"abstract-title\" style=\"text-align: justify;\">Abstract<\/h2>\n<section class=\"abstract\">\n<p style=\"text-align: justify;\">Numerous proteins require a metallic cofactor for their function. In plastids, the maturation of iron-sulfur (Fe-S) proteins necessitates a complex assembly machinery. We focused on <em>Arabidopsis thaliana<\/em> NFU1, NFU2 and NFU3, which participate in the late maturation steps. According to the strong photosynthesis defects observed in <em>high chlorophyll fluorescence 101<\/em> (<em>hcf101)<\/em>, <em>nfu2<\/em>and <em>nfu3<\/em> plants, we determined that NFU2 and NFU3, but not NFU1, act immediately upstream from HCF101 for the maturation of [Fe<sub>4<\/sub>S<sub>4<\/sub>]-containing photosystem I subunits. An additional function of NFU2 in the maturation of the [Fe<sub>2<\/sub>S<sub>2<\/sub>] cluster of a dihydroxyacid dehydratase was obvious from the accumulation of precursors of the branched-chain amino acid synthesis pathway in <em>nfu2<\/em> roots and the rescue of the primary root growth defect by supplying branched-chain amino acids. The absence of NFU3 in roots precluded any compensation. Overall, unlike their eukaryotic and prokaryotic counterparts, which are specific of [Fe<sub>4<\/sub>S<sub>4<\/sub>] proteins, NFU2 and NFU3 contribute to the maturation of both [Fe<sub>2<\/sub>S<sub>2<\/sub>] and [Fe<sub>4<\/sub>S<sub>4<\/sub>] proteins either as a relay in conjunction with other proteins such as HCF101 or by directly delivering Fe-S clusters to client proteins. Considering the low number of Fe-S cluster transfer proteins relative to final acceptors, additional targets probably await identification.<\/p>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>The iron-sulfur protein NFU2 plays a predominant role in branched-chain amino acid synthesis in Arabidopsis roots\u00a0B Touraine, F Vignols, J Przybyla-Toscano, T Ischebeck, T Dhalleine, &#8230; Journal of experimental botany Abstract Numerous proteins require a metallic cofactor for their function. &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=9531\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-9531","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/9531","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9531"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/9531\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9531"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9531"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9531"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}