{"id":9540,"date":"2019-03-05T10:54:08","date_gmt":"2019-03-05T10:54:08","guid":{"rendered":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=9540"},"modified":"2019-03-05T10:55:13","modified_gmt":"2019-03-05T10:55:13","slug":"article-new-phytologist-17","status":"publish","type":"post","link":"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=9540","title":{"rendered":"Article: New Phytologist"},"content":{"rendered":"<p><a class=\"gsc_a_at\" href=\"https:\/\/nph.onlinelibrary.wiley.com\/doi\/abs\/10.1111\/nph.15762\" data-href=\"\/citations?view_op=view_citation&amp;hl=fr&amp;user=szYQqz4AAAAJ&amp;sortby=pubdate&amp;citation_for_view=szYQqz4AAAAJ:HDshCWvjkbEC\">A two genes-for-one gene interaction between Leptosphaeria maculans Brassica napus.\u00a0<\/a>Y Petit-Houdenot, A Degrave, M Meyer, F Blaise, B Ollivier, CL Marais, &#8230; The New phytologist<\/p>\n<h1 class=\"article-section__header main abstractlang_en main\" style=\"text-align: justify;\">Summary<\/h1>\n<div class=\"article-section__content en main\">\n<p style=\"text-align: justify;\">Interactions between <i>Leptosphaeria maculans<\/i>, causal agent of stem canker of oilseed rape, and its Brassica hosts are models of choice to explore the multiplicity of \u201cgene\u2010for\u2010gene\u201d complementarities and how they diversified to increased complexity in the course of plant\u2010pathogen co\u2010evolution. Here, we comfort this postulate by investigating the <i>AvrLm10<\/i> avirulence that induces a resistance response when recognized by the <i>Brassica nigra<\/i> resistance gene <i>Rlm10<\/i>.<\/p>\n<p style=\"text-align: justify;\">Using genome\u2010assisted map\u2010based cloning, we identified and cloned two <i>AvrLm10<\/i> candidates as two genes in opposite transcriptional orientation located in a subtelomeric repeat\u2010rich region of the genome. The <i>AvrLm10<\/i>genes encode small secreted proteins and show expression profiles <i>in planta<\/i>similar to those of all <i>L. maculans<\/i> avirulence genes identified so far.<\/p>\n<p style=\"text-align: justify;\">Complementation and silencing assays indicated that both genes are necessary to trigger <i>Rlm10<\/i> resistance. Three assays for protein\u2010protein interactions showed that the two AvrLm10 proteins physically interact <i>in vitro<\/i>and <i>in planta<\/i>.<\/p>\n<p style=\"text-align: justify;\">Some avirulence genes are recognized by two distinct resistance genes and some avirulence genes hide the recognition specificities of another. Our <i>L. maculans<\/i> model illustrates an additional case where two genes located in opposite transcriptional orientation are necessary to induce resistance. Interestingly, orthologues exist for both <i>L. maculans<\/i> genes in other phytopathogenic species, with a similar genome organization, which may point to an important conserved effector function linked to heterodimerization of the two proteins.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>A two genes-for-one gene interaction between Leptosphaeria maculans Brassica napus.\u00a0Y Petit-Houdenot, A Degrave, M Meyer, F Blaise, B Ollivier, CL Marais, &#8230; The New phytologist Summary Interactions between Leptosphaeria maculans, causal agent of stem canker of oilseed rape, and its &hellip; <a href=\"https:\/\/mycor.iam.inrae.fr\/IAM\/?p=9540\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-9540","post","type-post","status-publish","format-standard","hentry","category-academic-productions"],"_links":{"self":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/9540","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9540"}],"version-history":[{"count":0,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=\/wp\/v2\/posts\/9540\/revisions"}],"wp:attachment":[{"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9540"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9540"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mycor.iam.inrae.fr\/IAM\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9540"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}