Career
I obtained my PhD in forest biology in November 2007. My Ph.D. focused on the study of molecular mechanisms involved in nitrogen uptake and remobilization in poplar. During my post-doc, my research was mainly focused on the study of the functions of glutaredoxins and their role in the biogenesis of iron-sulfur clusters. In September 2013, I was recruited as an associate professor at the University of Lorraine in the team Stress Response and Redox Regulation of the UMR 1136 UL/INRAE Tree/Microbres Interactions. My research activities are based on three main themes. The first one concerns the redox regulation of proteins in plants and the study of proteins of the glutaredoxin family (GRXs) which are oxidoreductases present in most of prokaryotes and eukaryotes, except for some bacteria and archaea. They are involved in the maintenance of the redox homeostasis of the cell through the reduction of disulfide bridges and more specifically of glutathionylated proteins. In higher plants, there are about thirty isoforms of GRXs. To date, less than ten of these proteins have been characterized in detail. The second major research theme concerns the study of the biogenesis of iron-sulfur clusters (Fe-S). Iron is an essential element for the synthesis of metalloproteins and in particular Fe-S proteins which are involved in vital processes such as photosynthesis, sulfur and nitrogen assimilation. Despite the importance of Fe-S proteins in plant function, current knowledge on Fe-S cluster biogenesis is mainly focused on the identification of the different components of this process, but the role of several of them is still undefined. On the other hand, the relationships between each of the components but also their specificities with respect to their target proteins are also unknown, as well as the underlying molecular mechanisms involved in the functioning and regulation of Fe-S cluster biogenesis. The third theme of my research concerns the molecular mechanisms involved in the mobilization and transfer of sulfur in plants. This theme concerns in particular the study of the role of sulfurtransferases (STRs) in these processes, knowing that these rhodanese domain containing enzymes catalyze the transfer of a sulfur atom from a donor to a nucleophilic acceptor, and that few data exist in plants. I obtained my habilitation in December 2020 and I was appointed as a junior member of the Institut Universitaire de France (IUF) for five years starting October 1, 2021.
Researcher IDs
Publications
2023
P57. Caubrière D, Moseler A, Rouhier N, Couturier J (2023) Diversity and roles of cysteine desulfurases in photosynthetic organisms. Journal of Experimental Botany 74:3345-3360. doi: 10.1093/jxb/erad065.
2022
P56. Christ L, Couturier J, Rouhier N (2022) Relationships between the Reversible Oxidation of the Single Cysteine Residue and the Physiological Function of the Mitochondrial Glutaredoxin S15 from Arabidopsis thaliana. Antioxidants 12:102. doi.org/10.3390/antiox12010102.
P55. de Bont L, Donnay N, Couturier J, Rouhier N (2022) Redox regulation of enzymes involved in sulfate assimilation and in the synthesis of sulfur-containing amino acids and glutathione in plants. Frontiers in Plant Science 13:958490. doi: 10.3389/fpls.2022.958490.
P54. Selles B, Moseler A, Caubrière D, Sun SK, Ziesel M, Dhalleine T, Hériché M, Wirtz M, Rouhier N, Couturier J (2022) The cytosolic Arabidopsis thaliana cysteine desulfurase ABA3 delivers sulfur to the sulfurtransferase STR18. The Journal of Biological Chemistry 298:101749. doi: 10.1016/j.jbc.2022.101749.
P53. Zannini F, Mathiot S, Couturier J, Didierjean C, Rouhier N (2022) Structural Insights into a Fusion Protein between a Glutaredoxin-like and a Ferredoxin-Disulfide Reductase Domain from an Extremophile Bacterium. Inorganics 10:24. doi: 10.3390/inorganics10020024.
2021
P52. Selles B, Dhalleine T, Boutilliat A, Rouhier N, Couturier J (2021) A Redox-Sensitive Cysteine Is Required for PIN1At Function. Frontiers in Plant Science 12:735423. doi: 10.3389/fpls.2021.735423
P51. Roret T, Zhang B, Moseler A, Dhalleine T, Gao XH, Couturier J, Lemaire SD, Didierjean C, Johnson MK, Rouhier N (2021) Atypical Iron-Sulfur Cluster Binding, Redox Activity and Structural Properties of Chlamydomonas reinhardtii Glutaredoxin 2. Antioxidants 10:803. doi: 10.3390/antiox10050803.
P50. Przybyla-Toscano J, Couturier J, Remacle C, Rouhier N (2021) Occurrence, Evolution and Specificities of Iron-Sulfur Proteins and Maturation Factors in Chloroplasts from Algae. International Journal of Molecular Sciences 22:3175. doi: 10.3390/ijms22063175.
P49. Moseler A, Dhalleine T, Rouhier N, Couturier J (2021) Arabidopsis thaliana 3-mercaptopyruvate sulfurtransferases interact with and are protected by reducing systems. The Journal of Biological Chemistry 296:100429 doi: 10.1016/j.jbc.2021.100429.
2020
P48. Azam T, Przybyla-Toscano J, Vignols F, Couturier J, Rouhier N, Johnson MK (2020) The Arabidopsis Mitochondrial Glutaredoxin GRXS15 Provides [2Fe-2S] Clusters for ISCA-Mediated [4Fe-4S] Cluster Maturation. International Journal of Molecular Sciences. 21:9237. doi: 10.3390/ijms21239237.
P47. Azam T, Przybyla-Toscano J, Vignols F, Couturier J, Rouhier N, Johnson MK. [4Fe-4S] cluster trafficking mediated by Arabidopsis mitochondrial ISCA and NFU proteins (2020) The Journal of Biological Chemistry 295:18367-18378. doi: 10.1074/jbc.RA120.015726.
P46. Roret T, Alloing G, Girardet JM, Perrot T, Dhalleine T, Couturier J, Frendo P, Didierjean C, Rouhier N (2020) Sinorhizobium meliloti YrbA binds divalent metal cations using two conserved histidines. Bioscience Reports, 40:BSR20202956. doi: 10.1042/BSR20202956.
P45. Berger N, Vignols F, Przybyla-Toscano J, Roland M, Rofidal V, Touraine B, Zienkiewicz K, Couturier J, Feussner I, Santoni V, Rouhier N, Gaymard F, Dubos C (2020) Identification of client iron-sulfur proteins of the chloroplastic NFU2 transfer protein in Arabidopsis thaliana. Journal of Experimental Botany 71:4171-4187 doi: 10.1093/jxb/eraa166.
P44. Roland M, Przybyla-Toscano J, Vignols F, Berger N, Azam T, Christ L, Santoni V, Wu HC, Dhalleine T, Johnson MK, Dubos C, Couturier J, Rouhier N (2020) The plastidial Arabidopsis thaliana NFU1 protein binds and delivers [4Fe-4S] clusters to specific client proteins. The Journal of Biological Chemistry 295:1727-1742. doi:10.1074/jbc.RA119.011034.
P43. Moseler A, Selles B, Rouhier N, Couturier J (2020) Novel insights into the diversity of the sulfurtransferase family in photosynthetic organisms with emphasis on oak. doi: 10.1111/nph.15870.
2019
P42. Rey P, Taupin-Broggini M, Couturier J, Vignols F, Rouhier N (2019) Is there a role for glutaredoxins and BOLAs in the perception of the cellular iron status in plants? Frontiers in Plant Science. 10:712. doi: 10.3389/fpls.2019.00712.
P41. Selles B, Moseler A, Rouhier N, Couturier J (2019) Rhodanese domain-containing sulfurtransferases: multifaceted proteins involved in sulfur trafficking in plants. Journal of Experimental Botany. 70:4139-4154. doi: 10.1093/jxb/erz213.
P40 Touraine B, Vignols F, Przybyla-Toscano J, Ischebeck T, Dhalleine T, Wu HC, Magno C, Berger N, Couturier J, Dubos C, Feussner I, Caffarri S, Havaux M, Rouhier N, Gaymard F (2019) The iron-sulfur protein NFU2 plays a predominant role in branched-chain amino acid synthesis in Arabidopsis roots. Journal of Experimental Botany. 70:1875-1889. doi: 10.1093/jxb/erz050.
P39 Zannini F, Moseler A, Bchini R, Dhalleine T, Meyer AM, Rouhier N, Couturier J (2019) The thioredoxin-mediated recycling of Arabidopsis thaliana GRXS16 relies on a conserved C-terminal cysteine. Biochimica and Biophysica Acta General Subjects 1863:426-436. doi: 10.1016/j.bbagen.2018.11.014.
2018
P38. Zannini F, Roret T, Przybyla-Toscano J, Dhalleine T, Rouhier N, Couturier J (2018) Mitochondrial Arabidopsis thaliana TRXo Isoforms Bind an Iron-Sulfur Cluster and Reduce NFU Proteins In Vitro. Antioxidants (Basel). 7 pii: E142. doi: 10.3390/antiox7100142.
P37. Gao H, Azam T, Randeniya S, Couturier J, Rouhier N, Johnson MK (2018) Function and maturation of the Fe-S center in dihydroxyacid dehydratase from Arabidopsis. The Journal of Biological Chemistry 293:4422-4433. doi: 10.1074/jbc.RA117.001592.
P36. Przybyla-Toscano J, Roland M, Gaymard F, Couturier J, Rouhier N (2018) Roles and maturation of iron-sulfur proteins in plastids. Journal of Biological Inorganic Chemistry. doi: 10.1007/s00775-018-1532-1
2017
P35. Zannini F, Couturier J, Keech O, Rouhier N (2017) In Vitro Alkylation Methods for Assessing the Protein Redox State. Methods in Molecular Biology 1653:51-64. doi: 10.1007/978-1-4939-7225-8_4.
P34. Przybyla-Toscano J, Roret T, Couturier J, Rouhier N (2017) FeS Cluster Assembly: Role of Monothiol Grxs and Nfu Proteins. Encyclopedia of Inorganic and Bioinorganic Chemistry, Metalloprotein Active Site Assembly. doi: 10.1002/9781119951438.eibc2470.
P33. Selles B, Zannini F, Couturier J, Jacquot JP, Rouhier N (2017) Atypical protein disulfide isomerases (PDI): Comparison of the molecular and catalytic properties of poplar PDI-A and PDI-M with PDI-L1A. PLoS One 12:e0174753. doi: 10.1371/journal.pone.0174753.
P32. Ribeiro CW, Baldacci-Cresp F, Pierre O, Larousse M, Benyamina S, Lambert A, Hopkins J, Castella C, Cazareth J, Alloing G, Boncompagni E, Couturier J, Mergaert P, Gamas P, Rouhier N, Montrichard F, Frendo P (2017) Regulation of Differentiation of Nitrogen-Fixing Bacteria by Microsymbiont Targeting of Plant Thioredoxin s1. Current Biology 27:250-256. doi: 10.1016/j.cub.2016.11.013.
2016
P31. Gütle DD, Roret T, Müller SJ, Couturier J, Lemaire SD, Hecker A, Dhalleine T, Buchanan BB, Reski R, Einsle O, Jacquot JP (2016) Chloroplast FBPase and SBPase are thioredoxin-linked enzymes with similar architecture but different evolutionary histories. Proceedings of the National Academy of Sciences USA 113: 6779-6784. doi: 10.1073/pnas.1606241113.
P30. Jacquot JP, Couturier J, Didierjean C, Gelhaye E, Morel-Rouhier M, Hecker A, Plomion C, Gütle DD, Rouhier N (2016) Structural and functional characterization of tree proteins involved in redox regulation: a new frontier in forest science. Annals of Forest Science 73:119-134. doi: 10.1007/s13595-014-0442-9
2015
P29. Rouhier N, Cerveau D, Couturier J, Reichheld JP, Rey P (2015) Involvement of thiol-based mechanisms in plant development. Biochimica and Biophysica Acta 1850:1479-1496. doi: 10.1016/j.bbagen.2015.01.023.
P28. Couturier J, Przybyla-Toscano J, Roret T, Didierjean C, Rouhier N (2015) The roles of glutaredoxins ligating Fe-S clusters: Sensing, transfer or repair functions? Biochimica and Biophysica Acta 1853:1513-1527. doi: 10.1016/j.bbamcr.2014.09.018.
2014
P27. Roret T, Pégeot H, Couturier J, Mulliert G, Rouhier N, Didierjean C (2014) X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana. Acta Crystallographica section F Structural Biology Communications 70:1180-1185. doi: 10.1107/S2053230X14017026.
P26. Roret T, Tsan P, Couturier J, Zhang B, Johnson MK, Rouhier N, Didierjean C (2014) Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes. The Journal of Biological Chemistry 289:24588-24598. doi: 10.1074/jbc.M114.572701.
P25. Dhalleine T, Rouhier N, Couturier J (2014) Putative roles of glutaredoxin-BolA holo-heterodimers in plants. Plant Signaling and Behaviour 9:e28564.
P24. Couturier J, Wu HC, Dhalleine T, Pégeot H, Sudre D, Gualberto JM, Jacquot JP, Gaymard F, Vignols F, Rouhier N (2014) Monothiol Glutaredoxin-BolA Interactions: Redox Control of Arabidopsis thaliana BolA2 and SufE1. Molecular Plant 7:187-205. doi: 10.1093/mp/sst156.
2013
P23. Couturier J, Jacquot JP, Rouhier N (2013) Toward a refined classification of class I dithiol glutaredoxins from poplar: biochemical basis for the definition of two subclasses. Frontiers in Plant Science 4:518. doi: 10.3389/fpls.2013.00518.
P22. Gao H, Subramanian S, Couturier J, Naik SG, Kim SK, Leustek T, Knaff DB, Wu HC, Vignols F, Huynh BH, Rouhier N, Johnson MK (2013) Arabidopsis thaliana Nfu2 accommodates [2Fe-2S] or [4Fe-4S] clusters and is competent for in vitro maturation of chloroplast [2Fe-2S] and [4Fe-4S] cluster-containing proteins. Biochemistry 52:6633-6645. doi: 10.1021/bi4007622.
P21. Zhang B, Bandyopadhyay S, Shakamuri P, Naik SG, Huynh BH, Couturier J, Rouhier N, Johnson MK (2013) Monothiol glutaredoxins can bind linear [Fe3S4]+ and [Fe4S4]2+ clusters in addition to [Fe2S2]2+ clusters: spectroscopic characterization and functional implications. J Am Chem Soc. 135:15153-15164. doi: 10.1021/ja407059n.
P20. Couturier J, Touraine B, Briat JF, Gaymard F, Rouhier N (2013) The iron-sulfur cluster assembly machineries in plants: current knowledge and open questions. Frontiers in Plant Science 4:259. doi: 10.3389/fpls.2013.00259.
P19. Couturier J, Chibani K, Jacquot JP, Rouhier N (2013) Cysteine-based redox regulation and signaling in plants. Frontiers in Plant Science 4:105. doi: 10.3389/fpls.2013.00105.
P18. Mapolelo D, Zhang B, Randeniya S, Albetel AN, Li H, Couturier J, Rouhier N, Outten CE, Johnson MK (2013) Monothiol glutaredoxins and A-type proteins: Partners in Fe-S cluster trafficking. Dalton Transactions 42:3107-3115. doi: 10.1039/c2dt32263c.
P17. Couturier J, Prosper P, Winger AM, Hecker A, Hirasawa M, Knaff D, Gans P, Jacquot JP, Navaza A, Haouz A, Rouhier N (2013) In the absence of thioredoxins, what are the reductants for peroxiredoxins in Thermotoga maritima? Antioxidants and Redox Signaling 18:1613-1622. doi: 10.1089/ars.2012.4739.
P16. Benyamina SM, Baldacci-Cresp F, Couturier J, Chibani K, Hopkins J, Bekki A, de Lajudie P, Rouhier N, Jacquot JP, Alloing G, Puppo A, Frendo P (2013) Two Sinorhizobium meliloti glutaredoxins regulate iron metabolism and symbiotic bacteroid differentiation. Environmental Microbiology 15:795-810. doi: 10.1111/j.1462-2920.2012.02835.x.
2012
P15. Couturier J, Vignols F, Jacquot JP, Rouhier N (2012) Glutathione- and glutaredoxin-dependent reduction of methionine sulfoxide reductase A. FEBS Letters 586: 3894-3899. doi: 10.1016/j.febslet.2012.09.020.
P14. Bedhomme M, Adamo M, Marchand CH, Couturier J, Rouhier N, Lemaire SD, Zaffagnini M, Trost P (2012) Glutathionylation of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the model plant Arabidopsis thaliana is reversed by both glutaredoxins and thioredoxins in vitro. Biochemical Journal. 445: 337-347. doi: 10.1042/BJ20120505.
P13. Zaffagnini M, Bedhomme M, Marchand CH, Couturier J, Gao XH, Rouhier N, Trost P, Lemaire SD (2012) Glutaredoxin S12: unique properties for redox signaling. Antioxidants and Redox Signaling 16: 17-32. doi: 10.1089/ars.2011.3933.
2011
P12. Couturier J, Ströher E, Albetel AN, Roret T, Muthuramalingam M, Tarrago L, Seidel T, Tsan P, Jacquot JP, Johnson MK, Dietz KJ, Didierjean C, Rouhier N (2011) Arabidopsis chloroplastic glutaredoxin C5 as a model to explore molecular determinants for iron-sulfur cluster binding into glutaredoxins. The Journal of Biological Chemistry 286: 27515-27527.
2010
P11. Couturier J, Didierjean C, Jacquot JP, Rouhier N (2010) Engineered mutated glutaredoxins mimicking peculiar plant class III glutaredoxins bind iron-sulfur centers and possess reductase activity. Biochemical and Biophysical Research Communications 403: 435-441.
P10. Couturier J, Doidy J, Guinet F, Wipf D, Blaudez D, Chalot M (2010) Glutamine, arginine and the amino acid transporter Pt-CAT11 play important roles during senescence in poplar. Annals of Botany 105: 1159-1169.
P9. Couturier J, de Faÿ E, Fitz M, Wipf D, Blaudez D, Chalot M (2010) PtAAP11, a high affinity amino acid transporter specifically expressed in differentiating xylem cells of poplar. Journal of Experimental Botany 61: 1671-1682.
P8. Rouhier N, Couturier J, Johnson MK, Jacquot JP (2010) Glutaredoxins: roles in iron homeostasis. Trends in Biochemical Sciences 35: 43-52.
P7. Chibani K, Couturier J, Selles B, Jacquot JP, Rouhier N (2010) The chloroplastic thiol reducing systems: dual functions in the regulation of carbohydrate metabolism and regeneration of antioxidant enzymes, emphasis on the poplar redoxin equipment. Photosynthesis Research 104: 75-99.
2009
P6. Selles B, Rouhier N, Chibani K, Couturier J, Gama F, Jacquot JP (2009) Glutaredoxin: The Missing Link Between Thiol-Disulfide Oxidoreductases and Iron Sulfur Enzymes. Advances in Botanical Research 52: 405-436
P5. Couturier J, Jacquot JP, Rouhier N (2009) Evolution and diversity of glutaredoxins in photosynthetic organisms. Cellular and Molecular Life Sciences 66: 2539-2557.
P4. Couturier J1, Koh CS1, Zaffagnini M1, Winger A, Gualberto JM, Corbier C, Decottignies P, Jacquot JP, Lemaire SD, Didierjean C, Rouhier N (2009) Structure-function relationship of the chloroplastic GrxS12 with an atypical WCSYS active site. The Journal of Biological Chemistry 284: 9299-9310.
2007
P3. Couturier J, Montanini B, Martin F, Brun A, Blaudez D, Chalot M (2007) The expanded family of ammonium transporters in the perennial poplar plant. New Phytologist 174:137-150.
2006
P2. Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Dejardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjarvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leple JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouze P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D (2006) The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science 313: 596-604.
P1. Rouhier N, Couturier J, Jacquot JP (2006) Genome-wide analysis of plant glutaredoxin systems. Journal of Experimental Botany 57: 1685-96.
